FMR1 Knockout cell line (SH-SY5Y)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

FMR1 (fragile X messenger ribonucleoprotein 1) encodes an RNA-binding protein associated with polysomes and involved in mRNA trafficking from the nucleus to the cytoplasm. FMR1 participates in post-transcriptional RNA regulation and cytoplasmic mRNA handling, supporting the transport and utilization of mRNAs in protein synthesis.

The SH-SY5Y cell line is a globally recognized, thrice-cloned sub-line derived from the human neuroblastoma line SK-N-SH, established in 1970 from a bone marrow biopsy of a 4-year-old female patient. Characterized as an N-type neuroblastoma line carrying a definitive ALK mutation, this versatile model serves as a premier in vitro platform for neurobiology research, neurodegenerative disease modeling, and tyrosine kinase inhibitor validation. Biochemically, SH-SY5Y cells maintain active dopamine-beta-hydroxylase activity and possess the functional capacity to convert glutamate to the neurotransmitter GABA, while demonstrating reliable in vivo performance by forming tumors in nude mice within 3-4 weeks.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.