Fgf21 Knockout cell line (AML12)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

Fstl1 (follistatin-like 1) encodes a secreted protein predicted to bind calcium ions and heparin. It is associated with endothelial cell differentiation and migration, negative regulation of apoptosis, and maintenance of hematopoietic stem cell homeostasis. Fstl1 is expressed across multiple tissues, including the cardiovascular system, central nervous system, genitourinary system, and limbs. Its biological functions have also been investigated in the context of pulmonary emphysema.

AML12 (alpha mouse liver 12) is a mouse hepatocyte-derived cell line established from hepatocytes of a transgenic CD1 mouse expressing human transforming growth factor alpha (TGF-α). The cell line represents a non-tumorigenic liver cell model and is used for studies of hepatocyte biology, liver metabolism, and molecular mechanisms regulating hepatic function.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.