GSDME Knockout cell line (A2058) – Genotype Verified & Ready-to-use

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GSDME Knockout cell line (A2058)

Copy Product Info

Catalog#

YKO-H2416

Species

Human

Target Cell Line

A2058

Size

1*10^6

Gene

GSDME

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

GSDME (gasdermin E) encodes a protein whose expression has been detected in the fetal cochlea and that has been implicated in nonsyndromic hearing impairment. The available annotation does not establish a specific molecular function or cellular pathway for GSDME. Genetic alterations in GSDME are associated with hearing impairment, suggesting an important role in auditory system development or function, although the mechanisms connecting GSDME activity with cochlear physiology remain incompletely characterized.

The A2058 cell line was established from a lymph node metastasis of malignant melanoma in a 43-year-old male patient. The cells exhibit highly invasive properties and have been used as a source for studying proteins associated with cellular invasion, including type IV collagenase, tissue inhibitor of metalloproteinase-2 (TIMP-2), and autocrine motility factor. A2058 is used as a human melanoma model for investigating tumor invasion and metastasis-related mechanisms.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the GSDME gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into A2058 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, GSDME Knockout cell line (A2058) is expanded and cryopreserved for downstream applications.

Product Overview

GSDME Knockout cell line (A2058)

The GSDME Knockout cell line (A2058) is generated by targeted disruption of the GSDME gene in A2058 cells. This cell line is designed to investigate the role of GSDME in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

GSDME Knockout cell line (A2058)

Gene ID

1687

Catalog

YKO-H2416

Gene Name

GSDME

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adheren

Catalog Status

-

Culture method

90%DMEM+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1：2-1：3

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?