Gys1 Knockout cell line (CTX-TNA2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

H2-K1 (histocompatibility 2, K1, K region) encodes a major histocompatibility complex class I protein involved in peptide antigen presentation. It participates in the presentation of endogenous peptides through the TAP-dependent endoplasmic reticulum pathway, as well as the presentation of exogenous antigens via MHC class I. H2-K1 also contributes to the positive regulation of T cell-mediated cytotoxicity and localizes to the external surface of the plasma membrane.

A rat astrocyte cell line derived from primary astrocytes isolated from the frontal cortex of 1-day-old Sprague-Dawley rats and immortalized by transfection with the oncogenic early region of SV40. The cells are phenotypically similar to type 1 astrocytes, with 20% GFAP-positive staining and a beta-alanine-inhibitable high-affinity GABA uptake mechanism. Alpha-2-macroglobulin production is similar to that of primary astrocytes, whereas transferrin production is reduced.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.