HKDC1 Knockout cell line (SW620)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

HKDC1 (hexokinase domain containing 1) encodes a member of the hexokinase protein family that is involved in glucose metabolism. HKDC1 may contribute to cellular glucose utilization and metabolic regulation. Reduced expression has been associated with gestational diabetes mellitus, while elevated expression has been associated with poor prognosis in hepatocellular carcinoma. These observations suggest that HKDC1 may have roles in metabolic regulation and disease-associated cellular phenotypes, although its precise biochemical functions require further characterization.

SW620 is a human colorectal cancer cell line established from a lymph node metastasis of a 51-year-old male patient with colorectal adenocarcinoma. The cells produce low levels of carcinoembryonic antigen (CEA), exhibit tumorigenicity in nude mice, and display heterogeneous morphology. SW620 represents a metastatic colorectal cancer model and is used for studies of tumor progression, metastasis, and cancer-related signaling pathways.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.