TLR3 Knockout cell line (A-431)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

TLR3 (toll like receptor 3) encodes a Toll-like receptor that recognizes double-stranded RNA associated with viral infection. Activation of TLR3 promotes signaling through NF-kappaB and induces production of type I interferons, contributing to antiviral innate immune responses. The receptor is expressed in selected immune and other tissues and functions as a component of cellular pathogen-recognition mechanisms that detect viral nucleic acids.

The A-431 cell line is a highly characterized human epidermoid carcinoma model established from a solid vulvar tumor of an 85-year-old female patient. As part of a series of cell lines isolated and characterized by D. J. Giard, et al. referenced in image_2f3fe4.png, this adherent model is globally recognized for its exceptional receptor expression density. Biochemically, A-431 cells express exceptionally large numbers of functional epidermal growth factor (EGF) binding sites, making it a foundational platform for investigating EGFR tyrosine kinase signaling pathways and receptor endocytosis. Serving as a robust industry standard, it functions as an essential in vitro indicator cell line for anti-TGF binding assays, receptor-targeted oncology screening, and basic cell proliferation research.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.