IQCB1 Knockout cell line (AC16)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

IQCB1 (IQ motif containing B1) encodes a ciliary protein containing IQ motifs and a coiled-coil region that interacts with calmodulin and the retinitis pigmentosa GTPase regulator. IQCB1 localizes to primary cilia of renal epithelial cells and connecting cilia of photoreceptors, where it contributes to ciliary function. Through these interactions, IQCB1 participates in maintenance of specialized ciliary structures and signaling.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.