KCNE3 Knockout cell line (AC16)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

KCNE3 (potassium voltage-gated channel subfamily E regulatory subunit 3) encodes a type I transmembrane auxiliary subunit of voltage-gated potassium channels. KCNE3 associates with potassium channel alpha subunits and modulates channel gating kinetics and stability, thereby influencing membrane excitability and ion transport. The protein is prominently expressed in the kidney and may contribute to epithelial electrolyte transport and other potassium-dependent cellular processes. Genetic alterations in KCNE3 have also been associated with hypokalemic periodic paralysis.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.