KCNMA1 Knockout cell line (AC16)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

KCNMA1 (potassium calcium-activated channel subfamily M alpha 1) encodes the alpha subunit of the large-conductance calcium-activated potassium (BK) channel. This channel integrates intracellular calcium signals with membrane potential to regulate ion flux and cellular excitability. KCNMA1 contributes to physiological processes including smooth muscle contraction, neurotransmitter release, and neuronal excitability. Mutations in KCNMA1 are associated with several neurological disorders, including paroxysmal nonkinesigenic dyskinesia, idiopathic generalized epilepsy, and Liang-Wang syndrome.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.