LDHA Knockout cell line (MSTO-211H）
Red cotton OmniCell bank
Overview
Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.
LDHA (lactate dehydrogenase A) encodes the A subunit of lactate dehydrogenase, an enzyme that reversibly converts pyruvate to lactate while oxidizing NADH to NAD+. This reaction is a key step in anaerobic glycolysis and supports regeneration of NAD+ required for continued glycolytic flux. LDHA therefore contributes to cellular energy metabolism and maintenance of redox balance during conditions in which oxidative metabolism is limited.
MSTO-211H is a human malignant mesothelioma cell line established from pleural effusion of a 62-year-old male patient with biphasic mesothelioma who had not received prior therapy. The cell line represents a malignant mesothelioma model and is used for studies of mesothelial tumor biology, cancer progression, and therapeutic response mechanisms.
Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.
Product Overview
Key Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Quality Control Specifications
Knockout Region: E3
Reference Transcript: LDHA-205
Validation Method: PCR amplification+Sanger sequencing
Download STR ReportIntended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
Unpacking and Storage Instructions
Handling procedure
References
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