MZF1 Knockout cell line (BEAS-2B)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

MZF1 (myeloid zinc finger 1) encodes a zinc finger-containing transcription factor that binds sequence-specific DNA regions associated with RNA polymerase II-dependent transcription. MZF1 can regulate gene expression in both positive and negative directions and is capable of forming protein homodimers. Through its DNA-binding and transcriptional regulatory activities, MZF1 may influence cellular programs involved in proliferation, differentiation, and other processes controlled by gene expression. The protein is primarily localized to the nucleoplasm.

BEAS-2B is an immortalized human bronchial epithelial cell line established from normal bronchial epithelium obtained from non-cancerous individuals. The cells were immortalized using a replication-defective SV40/adenovirus 12 hybrid (Ad12SV40) and cloned. BEAS-2B cells express epithelial markers including keratins and SV40 T antigen and can undergo squamous differentiation in response to serum exposure. The cell line is widely used as a model for studying normal airway epithelial biology, respiratory infection mechanisms, epithelial differentiation, environmental toxicity, and carcinogenesis.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.