Gstk1 Knockout cell line (TM3)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

Originated from primary cultures of Leydig cell-enriched preparations obtained from the normal testes of 11-13 day old BALB/c nu/+ mice, this cell line maintains biological characteristics highly similar to primary Leydig cells. The non-tumourigenic line responds specifically to luteinizing hormone (LH) but not to follicle-stimulating hormone (FSH), demonstrating the functional capacity to metabolize cholesterol in the presence of LH. Consequently, it serves as a reliable and specialized in vitro model for studying testicular physiology, androgen biosynthesis, and reproductive endocrinology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.