NECTIN4 Knockout cell line (SW780)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

NECTIN4 (nectin cell adhesion molecule 4) encodes a single-pass transmembrane cell adhesion molecule of the nectin family. It mediates cell-cell adhesion through homophilic and heterophilic interactions and can undergo proteolytic cleavage to generate a soluble form. NECTIN4 therefore contributes to organization of epithelial and other cellular contacts and is relevant to studies of cell adhesion, membrane signaling, and tissue organization. Mutations in NECTIN4 cause ectodermal dysplasia-syndactyly syndrome type 1.

A human bladder cancer model, SW780 originated from a primary urinary bladder transitional cell carcinoma of a 53-year-old female. The cells are classified as a human bladder carcinoma cell line and retain epithelial characteristics associated with their tumor origin. SW780 has been characterized through genomic and transcriptomic analyses and is represented in major cancer cell line resources.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.