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NGLY1 Knockout cell line (NCI-H82)

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NGLY1 Knockout cell line (NCI-H82)
Copy Product Info
Catalog#
YKO-HA100042
Species
Human
Target Cell Line
NCI-H82
Size
1*10^6
Gene
NGLY1
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
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Overview

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

NGLY1 (N-glycanase 1) encodes an enzyme that removes N-linked glycans from glycoproteins by hydrolyzing N(4)-(acetyl-beta-D-glucosaminyl) asparagine residues. This activity contributes to the processing of glycoproteins and is associated with proteasome-mediated degradation of misfolded glycoproteins. NGLY1 therefore participates in cellular protein quality control and the disposal of aberrant glycoproteins.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.

KO Cell Construction Strategy
Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the NGLY1 gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into NCI-H82 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, NGLY1 Knockout cell line (NCI-H82) is expanded and cryopreserved for downstream applications.
KO Cell Construction Strategy

Product Overview

NGLY1 Knockout cell line (NCI-H82)
The NGLY1 Knockout cell line (NCI-H82) is generated by targeted disruption of the NGLY1 gene in NCI-H82 cells. This cell line is designed to investigate the role of NGLY1 in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.
KO Cell Line
NGLY1 Knockout cell line (NCI-H82)
Gene ID
55768
Catalog
YKO-HA100042
Gene Name
NGLY1
Product Category
Knockout(KO) Cell Line
Gene Information
NCBI
Species
Human
Mycoplasma test
Negative
Cell Morphology
Grow in clumps, suspension
Catalog Status
-
Culture method
90%RPMI-1640+10%FBS
Shipping
Cryopreserved Cells shipped in dry ice
Passage ratio
1:2-1:3
Storage Condition
Liquid nitrogen
Quality Control
All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.
Applications
For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test
None Detected.
2. Mycoplasma test
Negative.
3. Knockout Strategy

Targeted knockout region: EExon 3

Reference Transcript: NM_001145294

Frameshift Mutation Strategy

4. STR Authentication
Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?
If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:
(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.
(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.
Q2. What Information Is Required When Reporting KO Cell Product Issues?
Q3. How Should the KO cell Product Be Stored Upon Receipt?
Q4. Shipping Method
Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?

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