Name: Notum Knockout cell line (MC3T3-E1 Subclone 14)
SKU: 1*10^6
Price: 2380 USD
Availability: InStock

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Wild-type Cell Lines

Notum Knockout cell line (MC3T3-E1 Subclone 14)

Copy Product Info

Catalog#

YKO-M019

Species

Mouse

Target Cell Line

MC3T3-E1 Subclone 14

Size

1*10^6

Gene

Notum

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

NOX5 (NADPH oxidase 5) encodes an NADPH oxidase that generates superoxide anions and has proton channel activity. It contributes to reactive oxygen species production, proton transport across membranes, and endothelial cell proliferation. NOX5 localizes to the plasma membrane and endoplasmic reticulum, where it can contribute to cellular redox signaling.

The MC3T3-E1 cell line is a foundational mouse osteoblast model derived from the cranial calvaria of newborn C57BL/6 mice. As referenced in image_2ede08.png, a series of subclones were isolated from the cloned but phenotypically heterogeneous parental MC3T3-E1 line to select for varying degrees of osteoblast differentiation and mineralization potential. This adherent line serves as a gold-standard in vitro platform for studying bone morphogenesis, osteogenesis pathway kinetics, and transcriptional regulation during matrix calcification.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the Notum gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into MC3T3-E1 Subclone 14 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, Notum Knockout cell line (MC3T3-E1 Subclone 14) is expanded and cryopreserved for downstream applications.

Product Overview

Notum Knockout cell line (MC3T3-E1 Subclone 14)

The Notum Knockout cell line (MC3T3-E1 Subclone 14) is generated by targeted disruption of the Notum gene in MC3T3-E1 Subclone 14 cells. This cell line is designed to investigate the role of Notum in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

Notum Knockout cell line (MC3T3-E1 Subclone 14)

Gene ID

77583

Catalog

YKO-M019

Gene Name

Notum

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Mouse

Mycoplasma test

Negative

Cell Morphology

Fibroblast-like, adherent

Catalog Status

-

Culture method

90% Alpha MEM+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:3~1:5

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: E1

Reference Transcript: Notum-202

Frameshift Mutation Strategy

4. STR Authentication

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?