OAT Knockout cell line (NCI-H82)
Red cotton OmniCell bank
Overview
Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.
OAT (ornithine aminotransferase) encodes a mitochondrial enzyme that catalyzes reactions involving ornithine and arginine metabolism. It contributes to the metabolic pathway connecting arginine and ornithine with the production of glutamate and GABA, linking amino acid metabolism with neurotransmitter biosynthesis. OAT activity is therefore important for mitochondrial amino acid metabolism and cellular processes involving these metabolic intermediates.
Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.
Product Overview
Key Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Quality Control Specifications
Targeted knockout region: EExon 3
Reference Transcript: NM_001322965
Frameshift Mutation Strategy
Download STR ReportIntended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
References
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