OS9 Knockout cell line (NCI-H82)
Red cotton OmniCell bank
Overview
Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.
OS9 (OS9 endoplasmic reticulum lectin) encodes an endoplasmic reticulum-associated protein that binds hypoxia-inducible factor 1 (HIF-1) and promotes degradation of one of its subunits. Through this activity, OS9 participates in regulation of HIF-1 stability and the cellular response to hypoxia. The protein may therefore contribute to regulation of hypoxia-associated processes and cellular adaptation to low-oxygen conditions.
Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.
Product Overview
Key Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Quality Control Specifications
Targeted knockout region: EExon 1
Reference Transcript: NM_001410980
Frameshift Mutation Strategy
Download STR ReportIntended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
References
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