PHKA1 Knockout cell line (AC16)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

PHKA1 (phosphorylase kinase regulatory subunit alpha 1) encodes the skeletal muscle alpha subunit of phosphorylase kinase, a multisubunit enzyme complex that regulates glycogen breakdown. The alpha subunit has a regulatory role within the complex, whose catalytic gamma subunit phosphorylates glycogen phosphorylase to promote glycogenolysis. PHKA1 therefore contributes to regulation of muscle glycogen metabolism, and defects in this gene cause X-linked muscle glycogen storage disease.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.