PIGA Knockout cell line (AC16)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

PIGA (phosphatidylinositol glycan anchor biosynthesis class A) encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol, the first intermediate in biosynthesis of glycosylphosphatidylinositol anchors. GPI anchors attach numerous proteins to the cell surface and are important for proper membrane protein localization and function. PIGA is therefore essential for GPI anchor biosynthesis and cell-surface protein expression. Acquired mutations in PIGA are associated with paroxysmal nocturnal hemoglobinuria.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.