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Red cotton OmniCell bank

8000+
Gene Knockout Cell Lines
2000+
Luc/EGFP/Cas9 Tool Cell Lines
1000+
Wild-type Cell Lines
Copy Product Info
Catalog#
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Species
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Target Cell Line
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Size
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Gene
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Storage conditions
Shipped on dry ice; Store in liquid nitrogen

Overview

Gene knockout cell lines are genetically engineered using CRISPR-Cas9 technology to precisely disrupt target genes at the genomic level, resulting in complete loss of gene function. These cell models are widely used in gene function studies, signaling pathway analysis, disease mechanism research, and drug target validation. Ubigene has developed a portfolio of over 8,000 gene knockout cell lines, covering major cell types and high-interest targets, enabling rapid support for diverse research needs.

is generated by targeted disruption of the gene in cells. This cell line is designed to facilitate investigation of function in cellular processes, gene regulation, and its involvement in disease-related pathways.The gene knockout cell is validated by Sanger sequencing, and each clone is accompanied by STR authentication documentation. The product is delivered as a homozygous single-cell clone with stable passaging capability. It is guaranteed to be mycoplasma-free and exhibits high viability. In addition, comprehensive technical support and complimentary cell culture guidance are provided throughout use.

KO Cell Construction Strategy
Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, is expanded and cryopreserved for downstream applications.
KO Cell Construction Strategy

Product Overview

The is generated by targeted disruption of the gene in cells. This cell line is designed to investigate the role of in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.
KO Cell Line
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Gene ID
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Catalog
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Gene Name
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Product Category
Knockout(KO) Cell Line
Gene Information
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Species
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Mycoplasma test
Negative
Cell Morphology
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Catalog Status
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Culture method
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Shipping
Cryopreserved Cells shipped in dry ice
Passage ratio
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Storage Condition
Liquid nitrogen
Quality Control
All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.
Applications
For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test
None Detected.
2. Mycoplasma test
Negative.

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

References

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?
If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:
(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.
(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.
Q2. What Information Is Required When Reporting KO Cell Product Issues?
Q3. How Should the KO cell Product Be Stored Upon Receipt?
Q4. Shipping Method
Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?

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