PPP2R5C Knockout cell line (SH-SY5Y)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

PPP2R5C (protein phosphatase 2 regulatory subunit B'gamma) encodes a regulatory subunit of protein phosphatase 2A (PP2A). It modulates PP2A substrate specificity and catalytic activity, contributing to regulation of cellular processes including growth and division.

The SH-SY5Y cell line is a globally recognized, thrice-cloned sub-line derived from the human neuroblastoma line SK-N-SH, established in 1970 from a bone marrow biopsy of a 4-year-old female patient. Characterized as an N-type neuroblastoma line carrying a definitive ALK mutation, this versatile model serves as a premier in vitro platform for neurobiology research, neurodegenerative disease modeling, and tyrosine kinase inhibitor validation. Biochemically, SH-SY5Y cells maintain active dopamine-beta-hydroxylase activity and possess the functional capacity to convert glutamate to the neurotransmitter GABA, while demonstrating reliable in vivo performance by forming tumors in nude mice within 3-4 weeks.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.