PRKD3 Knockout cell line (AC16)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

PRKD3 (protein kinase D3) encodes a serine/threonine protein kinase of the protein kinase D family that binds diacylglycerol and phorbol esters. Its regulatory region contains cysteine-rich zinc-finger motifs and a pleckstrin homology domain, while the C-terminal region contains the catalytic kinase domain. PRKD3 participates in signaling processes regulating epithelial barrier formation, cancer cell growth, and vesicle trafficking.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.