SLC7A11 Knockout cell line （Caco-2）

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.