TAX1BP3 Knockout cell line (BEAS-2B)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

TAX1BP3 (Tax1 binding protein 3) encodes a PDZ domain-containing protein that mediates protein-protein interactions involved in cellular signaling, adhesion, and protein organization. TAX1BP3 interacts with signaling proteins including RhoA and glutaminase and functions as a negative regulator of Wnt/beta-catenin signaling. It also regulates the localization and density of inwardly rectifying potassium channels and contributes to cellular proliferation, development, stress responses, and polarization.

BEAS-2B is an immortalized human bronchial epithelial cell line established from normal bronchial epithelium obtained from non-cancerous individuals. The cells were immortalized using a replication-defective SV40/adenovirus 12 hybrid (Ad12SV40) and cloned. BEAS-2B cells express epithelial markers including keratins and SV40 T antigen and can undergo squamous differentiation in response to serum exposure. The cell line is widely used as a model for studying normal airway epithelial biology, respiratory infection mechanisms, epithelial differentiation, environmental toxicity, and carcinogenesis.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.