TMEM120A Knockout cell line (A-375)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

TMEM120A (transmembrane protein 120A) encodes a membrane-associated protein located in the endoplasmic reticulum and plasma membrane. It has coenzyme A-binding activity and is involved in antiviral innate immune responses, adipocyte differentiation, and protein complex oligomerization. TMEM120A therefore contributes to membrane-associated cellular regulation and metabolic or differentiation-related processes.

The A375 cell line is a highly characterized human malignant melanoma model derived from a 54-year-old female patient. As referenced in image_2f51f1.png, this adherent cell line demonstrates robust translational performance in in vivo xenograft studies, reliably producing rapidly growing amelanotic melanomas when inoculated into anti-thymocyte serum-treated NIH Swiss mice. Serving as a globally recognized preclinical oncology platform, it is extensively utilized for investigating melanoma disease progression, executing high-throughput screening of novel therapeutics, and studying targeted signaling pathways in skin cancers.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.