TMEM159 Knockout cell line (Caco-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

LDAF1 (lipid droplet assembly factor 1) encodes a protein involved in lipid droplet formation and is localized to the endoplasmic reticulum membrane and lipid droplets. LDAF1 contributes to the biogenesis and organization of lipid droplets, specialized organelles that store neutral lipids and regulate cellular lipid homeostasis. Through its association with the ER-lipid droplet interface, LDAF1 participates in cellular mechanisms controlling lipid storage and mobilization.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.