TRIB1 Knockout cell line (Hep G2)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

TRIB1 (tribbles pseudokinase 1) encodes a pseudokinase that functions as a regulator of protein signaling and transcriptional processes. TRIB1 interacts with mitogen-activated protein kinase kinases and transcription factors and can modulate protein kinase activity. It participates in signaling pathways including the JNK cascade and negatively regulates lipopolysaccharide-mediated signaling. Through these activities, TRIB1 contributes to regulation of intracellular signaling and inflammatory responses in both cytoplasmic and nuclear compartments.

A human liver tumor cell line established from the tumor tissue of a 15-year-old Caucasian male in 1975, originally described as a well-differentiated hepatocellular carcinoma and later reclassified by some sources as a hepatoblastoma. The cells produce multiple plasma proteins, including albumin, alpha-fetoprotein, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, and plasminogen. Hepatitis B virus surface antigens have not been detected, and the cells respond to stimulation with human growth hormone.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.