TRMT9B Knockout cell line (VERO C1008 (E6))

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

Trp53 (transformation related protein 53) encodes the tumor suppressor protein p53, a DNA-binding transcription factor that responds to diverse cellular stresses. It regulates target genes involved in cell-cycle arrest, apoptosis, senescence, DNA repair, and metabolic changes, helping prevent uncontrolled cell growth and tumor development. Trp53-deficient mice develop normally but are susceptible to spontaneous tumors. The gene has a single reported promoter in mice and produces several alternatively spliced transcripts, although the biological validity of some variants remains uncertain.

VERO C1008 (E6) is a subclone of the Vero76 cell line, which was originally derived from the kidney of an African green monkey. The cells retain susceptibility to a range of viruses and exhibit some degree of contact inhibition, making them suitable for supporting the growth and study of slowly replicating viruses. VERO C1008 (E6) is widely used in virology research, including viral propagation, infection studies, and evaluation of viral replication.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.