Tollip Knockout cell line (MPC-5)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

Tpm1 (tropomyosin 1) encodes a member of the tropomyosin protein family that forms heterodimers with a beta subunit and binds actin filaments in muscle and nonmuscle cells. In smooth and striated muscle, TPM1 contributes to calcium-dependent regulation of muscle contraction. In nonmuscle cells, it helps stabilize the actin cytoskeleton. Alternative splicing generates multiple transcript variants encoding distinct protein isoforms, supporting diverse functions across different cellular contexts.

MPC-5 is a mouse plasma cell tumor-derived cell line established from the Merwin plasma cell tumor-II carried in BALB/c mice. The cells retain plasma cell characteristics and produce fully assembled immunoglobulin G2b (IgG2b), as well as immunoglobulin-related subunits including light chain dimers and free light chains. MPC-5 is used as a model for studying plasma cell biology, immunoglobulin production, and antibody synthesis mechanisms.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.