Trib1 Knockout cell line (C2C12)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

The C2C12 cell line is a highly dynamic, rapidly proliferating subclone produced by H. Blau et al. from the parental mouse myoblast line originally established by D. Yaffe and O. Saxel from the normal adult leg muscle of the C3H mouse strain. Universally recognized as a premier in vitro model for studying myogenesis, skeletal muscle development, and cellular differentiation kinetics, this adherent line possesses exceptional multi-lineage plasticity. When incubated with horse serum at high confluency, C2C12 cells differentiate rapidly to form extensive, contracting multinucleated myotubes that express characteristic muscle-specific proteins; conversely, treating the culture with bone morphogenetic protein 2 (BMP-2) successfully redirects their lineage to differentiate into functional osteoblasts. To preserve this high-differentiation potency and prevent spontaneous fusion, cultures must be proactively seeded at a low density and strictly maintained at a sub-confluent state below 80% maximum density.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.