VHL Knockout cell line(HCT 116)
Red cotton OmniCell bank
Online inquireOverview
CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.
HCT 116 is a human colorectal carcinoma cell line established from a primary colon tumor of an adult male patient. The cells are tumorigenic in nude mice, form colonies in agarose, and carry a mutation in the RAS gene at codon 13. HCT 116 is widely used as a model for studying colorectal cancer biology, oncogenic signaling pathways, tumorigenesis, and anticancer drug responses.
Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.
Product Overview
Key Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Quality Control Specifications
Knockout Region: E1
Reference Transcript: VHL-201
Validation Method: PCR amplification+Sanger sequencing
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
Unpacking and Storage Instructions
Handling procedure
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