VPS41 Knockout cell line (AC16)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

VPS41 (VPS41 subunit of HOPS complex) encodes a component of the HOPS complex involved in vesicle-mediated protein sorting and intracellular membrane trafficking. VPS41 contributes to transport and fusion of vesicles, including trafficking from the Golgi toward lysosome-related compartments. Through its role in HOPS complex function, VPS41 supports organization of intracellular cargo within distinct membrane-bound organelles and contributes to vesicle fusion and delivery.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.