WASHC2A Knockout cell line (A-375)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

WASHC2A (WASH complex subunit 2A) encodes a component of the WASH complex that participates in endosomal protein localization. It has predicted phosphatidylinositol phosphate- and retromer-binding activities and is associated with the cytosol and nucleolus. WASHC2A contributes to membrane trafficking and endosomal organization through interactions with phosphoinositides and retromer-associated machinery.

The A375 cell line is a highly characterized human malignant melanoma model derived from a 54-year-old female patient. As referenced in image_2f51f1.png, this adherent cell line demonstrates robust translational performance in in vivo xenograft studies, reliably producing rapidly growing amelanotic melanomas when inoculated into anti-thymocyte serum-treated NIH Swiss mice. Serving as a globally recognized preclinical oncology platform, it is extensively utilized for investigating melanoma disease progression, executing high-throughput screening of novel therapeutics, and studying targeted signaling pathways in skin cancers.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.