ZBTB33 Knockout cell line (AC16)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZBTB33 (zinc finger and BTB domain containing 33) encodes a transcriptional regulator with bimodal DNA-binding specificity for methylated and non-methylated DNA sequences. The protein contains an N-terminal BTB domain and C-terminal zinc fingers and recruits the N-CoR corepressor complex to promote histone deacetylation and repressive chromatin formation. ZBTB33 can regulate transcription of target genes, including genes associated with Wnt signaling, through interactions with corepressor and catenin proteins.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.