ZBTB7B Knockout cell line (Caco-2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZBTB7B (zinc finger and BTB domain containing 7B) encodes a zinc finger transcription factor that regulates lineage commitment of immature T-cell precursors. It promotes commitment toward the CD4 T-cell lineage and, in its absence, favors CD8 lineage commitment. ZBTB7B also functions as a transcriptional repressor of type I collagen genes, linking its activity to both T-cell development and regulation of extracellular matrix-related gene expression.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.