ZC3H12A Knockout cell line (Jurkat, Clone E6-1) – Genotype Verified & Ready-to-use

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ZC3H12A Knockout cell line (Jurkat, Clone E6-1)

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Catalog#

YKO-HB60463

Species

Human

Target Cell Line

Jurkat, Clone E6-1

Size

1*10^6

Gene

ZC3H12A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZC3H12A (zinc finger CCCH-type containing 12A) encodes a CCCH-type zinc finger protein that has been described as an MCP1-induced transcriptional regulator. The protein has been implicated in activation of gene expression and induction of cardiomyocyte cell death, potentially through regulation of apoptosis-associated genes. Its biological activities suggest a role in transcriptional regulation and cellular survival, although the molecular mechanisms underlying these effects remain incompletely characterized.

A human T-cell line originating from the peripheral blood of a 14-year-old male with childhood T-cell acute lymphoblastic leukemia, Jurkat, Clone E6-1 represents a clone of the Jurkat-FHCRC derivative. The line is classified as a T-cell lymphoblast and has been characterized with genomic and proteomic studies. It is associated with precursor T-cell acute lymphoblastic leukemia and retains the biological characteristics of its leukemia-derived origin.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the ZC3H12A gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into Jurkat, Clone E6-1 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, ZC3H12A Knockout cell line (Jurkat, Clone E6-1) is expanded and cryopreserved for downstream applications.

Product Overview

ZC3H12A Knockout cell line (Jurkat, Clone E6-1)

The ZC3H12A Knockout cell line (Jurkat, Clone E6-1) is generated by targeted disruption of the ZC3H12A gene in Jurkat, Clone E6-1 cells. This cell line is designed to investigate the role of ZC3H12A in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

ZC3H12A Knockout cell line (Jurkat, Clone E6-1)

Gene ID

80149

Catalog

YKO-HB60463

Gene Name

ZC3H12A

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Lymphocyte-like,suspension

Catalog Status

-

Culture method

90%RPMI-1640+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:3-1:5

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 2

Reference Transcript: NM_025079

Frameshift Mutation Strategy

4. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?