ZC3H7A Knockout cell line (T24) – Genotype Verified & Ready-to-use

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ZC3H7A Knockout cell line (T24)

Copy Product Info

Catalog#

YKO-HA135242

Species

Human

Target Cell Line

T24

Size

1*10^6

Gene

ZC3H7A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZC3H7A (zinc finger CCCH-type containing 7A) encodes a CCCH-type zinc finger protein that binds miRNAs and participates in miRNA processing. It positively regulates post-transcriptional gene expression, making it relevant for studies of RNA biology, miRNA-mediated regulation, and gene expression control.

The T24 cell line was established in 1970 from a primary grade III transitional cell carcinoma (urothelial carcinoma) of the urinary bladder from an 81-year-old female patient. This human bladder carcinoma cell line is widely characterized and has been reported to carry HRAS and p53 mutations. T24 cells produce multiple cytokines, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and stem cell factor (SCF). The line is commonly used as a model for studying bladder cancer biology, oncogenic signaling pathways, cytokine regulation, and therapeutic responses in urothelial carcinoma.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the ZC3H7A gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into T24 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, ZC3H7A Knockout cell line (T24) is expanded and cryopreserved for downstream applications.

Product Overview

ZC3H7A Knockout cell line (T24)

The ZC3H7A Knockout cell line (T24) is generated by targeted disruption of the ZC3H7A gene in T24 cells. This cell line is designed to investigate the role of ZC3H7A in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

ZC3H7A Knockout cell line (T24)

Gene ID

29066

Catalog

YKO-HA135242

Gene Name

ZC3H7A

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adherent

Catalog Status

-

Culture method

90%RPMI-1640+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:3~1:4

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 5

Reference Transcript: NM_014153

Frameshift Mutation Strategy

4. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?