ZDHHC18 Knockout cell line (U-251 MG) – Genotype Verified & Ready-to-use

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ZDHHC18 Knockout cell line (U-251 MG)

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Catalog#

YKO-HA153596

Species

Human

Target Cell Line

U-251 MG

Size

1*10^6

Gene

ZDHHC18

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZDHHC18 (zDHHC palmitoyltransferase 18) encodes a protein-cysteine S-palmitoyltransferase that catalyzes protein S-palmitoylation. The enzyme is active in the Golgi apparatus and contributes to regulation of the cGAS-STING signaling pathway and innate immune responses. ZDHHC18 is therefore relevant to studies of protein lipid modification, membrane-associated enzyme activity, innate immune signaling, and regulation of cellular signaling pathways.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the ZDHHC18 gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into U-251 MG cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, ZDHHC18 Knockout cell line (U-251 MG) is expanded and cryopreserved for downstream applications.

Product Overview

ZDHHC18 Knockout cell line (U-251 MG)

The ZDHHC18 Knockout cell line (U-251 MG) is generated by targeted disruption of the ZDHHC18 gene in U-251 MG cells. This cell line is designed to investigate the role of ZDHHC18 in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

ZDHHC18 Knockout cell line (U-251 MG)

Gene ID

84243

Catalog

YKO-HA153596

Gene Name

ZDHHC18

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adherent

Catalog Status

-

Culture method

90% DMEM+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:2~1:4

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 1

Reference Transcript: NM_032283

Frameshift Mutation Strategy

4. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?