ZDHHC23 Knockout cell line (HK-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZDHHC23 (zDHHC palmitoyltransferase 23) encodes a predicted protein-cysteine S-palmitoyltransferase involved in protein palmitoylation and protein localization to the plasma membrane. The protein is predicted to localize to the Golgi membrane and to function in the Golgi apparatus and endoplasmic reticulum. Through palmitoylation of target proteins, ZDHHC23 may contribute to regulation of membrane-associated protein trafficking, localization, and function.

HK-2 (Human Kidney-2) is a well-characterized immortalized human kidney proximal tubular epithelial cell line derived from normal adult renal tissue. Transformed via the transduction of human papillomavirus type 16 (HPV-16) E6/E7 genes, HK-2 successfully achieves continuous in vitro proliferation while stably retaining the functional and phenotypic characteristics of primary proximal tubular epithelium. HK-2 cells maintain distinct brush border enzyme activities and exhibit physiologic responsiveness to cytokines and nephrotoxic compounds. Consequently, HK-2 serves as a globally accepted, standard human cell platform extensively utilized in in vitro nephrotoxicity screening, renal fibrosis modeling, tubular transport mechanism profiling, and preclinical drug safety evaluations.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.