ZER1 Knockout cell line (A-375)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZER1 (zyg-11 related cell cycle regulator) encodes a subunit of an E3 ubiquitin ligase complex that may participate in regulation of meiosis. The protein contains multiple leucine-rich repeat motifs, which may contribute to its interactions within ubiquitin-dependent regulatory complexes. ZER1 is relevant to studies of E3 ubiquitin ligase function, protein turnover, cell-cycle regulation, and meiotic cellular processes.

The A375 cell line is a highly characterized human malignant melanoma model derived from a 54-year-old female patient. As referenced in image_2f51f1.png, this adherent cell line demonstrates robust translational performance in in vivo xenograft studies, reliably producing rapidly growing amelanotic melanomas when inoculated into anti-thymocyte serum-treated NIH Swiss mice. Serving as a globally recognized preclinical oncology platform, it is extensively utilized for investigating melanoma disease progression, executing high-throughput screening of novel therapeutics, and studying targeted signaling pathways in skin cancers.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.