ZFAND5 Knockout cell line (Caco-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZFAND5 (zinc finger AN1-type containing 5) encodes a zinc finger-containing protein with predicted DNA-binding and zinc ion-binding activities. It is predicted to participate in regulation of processes including fibroblast migration and platelet-derived growth factor receptor signaling and is localized primarily to the cytoplasm. ZFAND5 is relevant to studies of zinc finger protein function, cellular signaling, cell migration, and regulation of gene expression.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.