ZFP36 Knockout cell line (THP-1)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZFP36 (ZFP36 zinc finger CCCH-type) encodes an RNA-binding regulatory protein that recognizes AU-rich regions in the 3′ untranslated regions of target mRNAs. It participates in regulation of gene expression and mRNA catabolism and responds to cytokine and epidermal growth factor signaling. Through sequence-specific RNA interactions, ZFP36 contributes to post-transcriptional control of gene expression and cellular responses to extracellular stimuli.

THP-1 is a human monocytic leukemia cell line established from the peripheral blood of a 1-year-old male patient with acute monocytic leukemia at relapse. The cells exhibit monocytic characteristics, including expression of Fc and complement receptors, lysozyme production, esterase activity, and phagocytic capacity. THP-1 cells can be differentiated into macrophage-like cells under appropriate stimulation and are widely used as a model for studying monocyte/macrophage biology, immune responses, inflammation, differentiation, and host–pathogen interactions.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.