ZFP36L1 Knockout cell line (HCT 116)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZFP36L1 (ZFP36 like 1 zinc finger CCCH-type) encodes a member of the TIS11 family of early-response proteins containing a putative CCCH-type zinc finger domain. It is induced by stimuli including phorbol esters and epidermal growth factor and is predicted to participate in regulation of cellular responses to growth factors. Its zinc finger-containing structure is consistent with a role in nucleic-acid-associated regulation, although the provided annotation does not establish detailed molecular mechanisms.

HCT 116 is a human colorectal carcinoma cell line established from a primary colon tumor of an adult male patient. The cells are tumorigenic in nude mice, form colonies in agarose, and carry a mutation in the RAS gene at codon 13. HCT 116 is widely used as a model for studying colorectal cancer biology, oncogenic signaling pathways, tumorigenesis, and anticancer drug responses.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.