ZFP57 Knockout cell line (HK-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZFP57 (ZFP57 zinc finger protein) encodes a KRAB domain-containing zinc finger protein that functions as a transcriptional regulator. ZFP57 is predicted to recognize specific DNA sequences and recruit regulatory complexes to influence gene expression and epigenetic states. Its zinc finger and KRAB domains support DNA binding and transcriptional repression, although the precise targets and mechanisms of ZFP57 regulation remain incompletely characterized.

HK-2 (Human Kidney-2) is a well-characterized immortalized human kidney proximal tubular epithelial cell line derived from normal adult renal tissue. Transformed via the transduction of human papillomavirus type 16 (HPV-16) E6/E7 genes, HK-2 successfully achieves continuous in vitro proliferation while stably retaining the functional and phenotypic characteristics of primary proximal tubular epithelium. HK-2 cells maintain distinct brush border enzyme activities and exhibit physiologic responsiveness to cytokines and nephrotoxic compounds. Consequently, HK-2 serves as a globally accepted, standard human cell platform extensively utilized in in vitro nephrotoxicity screening, renal fibrosis modeling, tubular transport mechanism profiling, and preclinical drug safety evaluations.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.