ZHX3 Knockout cell line (AC16)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZHX3 (zinc fingers and homeoboxes 3) encodes a transcriptional regulatory protein containing C2H2-type zinc fingers and multiple homeodomains. ZHX3 can form homodimers or heterodimers with ZHX1 and, in the nucleus, interacts with the A subunit of nuclear factor-Y. These interactions support a role as a transcriptional repressor, suggesting that ZHX3 contributes to regulation of gene expression through protein-protein interactions and DNA-associated transcriptional control.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.