ZKSCAN1 Knockout cell line (HK-2)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZKSCAN1 (zinc finger with KRAB and SCAN domains 1) encodes a member of the Kruppel C2H2-type zinc finger protein family that may function as a transcription factor. The protein has been reported to regulate expression of GABA type-A receptor genes in the brain. ZKSCAN1 can also produce stable circular RNAs, and its expression has been associated with gastric cancer cell migration and invasion.

HK-2 (Human Kidney-2) is a well-characterized immortalized human kidney proximal tubular epithelial cell line derived from normal adult renal tissue. Transformed via the transduction of human papillomavirus type 16 (HPV-16) E6/E7 genes, HK-2 successfully achieves continuous in vitro proliferation while stably retaining the functional and phenotypic characteristics of primary proximal tubular epithelium. HK-2 cells maintain distinct brush border enzyme activities and exhibit physiologic responsiveness to cytokines and nephrotoxic compounds. Consequently, HK-2 serves as a globally accepted, standard human cell platform extensively utilized in in vitro nephrotoxicity screening, renal fibrosis modeling, tubular transport mechanism profiling, and preclinical drug safety evaluations.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.