ZMIZ2 Knockout cell line (T24) – Genotype Verified & Ready-to-use

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ZMIZ2 Knockout cell line (T24)

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Catalog#

YKO-HA135289

Species

Human

Target Cell Line

T24

Size

1*10^6

Gene

ZMIZ2

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZMIZ2 (zinc finger MIZ-type containing 2) encodes a PIAS-like protein that interacts with nuclear hormone receptors. ZMIZ2 can interact with the androgen receptor and enhance androgen receptor-mediated transcription, indicating a role in regulation of hormone-responsive gene expression. ZMIZ2 knockout models are relevant to studies of nuclear receptor signaling, transcriptional regulation, and cellular responses to androgen-dependent signaling.

The T24 cell line was established in 1970 from a primary grade III transitional cell carcinoma (urothelial carcinoma) of the urinary bladder from an 81-year-old female patient. This human bladder carcinoma cell line is widely characterized and has been reported to carry HRAS and p53 mutations. T24 cells produce multiple cytokines, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and stem cell factor (SCF). The line is commonly used as a model for studying bladder cancer biology, oncogenic signaling pathways, cytokine regulation, and therapeutic responses in urothelial carcinoma.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the ZMIZ2 gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into T24 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, ZMIZ2 Knockout cell line (T24) is expanded and cryopreserved for downstream applications.

Product Overview

ZMIZ2 Knockout cell line (T24)

The ZMIZ2 Knockout cell line (T24) is generated by targeted disruption of the ZMIZ2 gene in T24 cells. This cell line is designed to investigate the role of ZMIZ2 in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

ZMIZ2 Knockout cell line (T24)

Gene ID

83637

Catalog

YKO-HA135289

Gene Name

ZMIZ2

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adherent

Catalog Status

-

Culture method

90%RPMI-1640+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:3~1:4

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 4

Reference Transcript: NM_001300959

Frameshift Mutation Strategy

4. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?