ZNF101 Knockout cell line (Hep G2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZNF101 (zinc finger protein 101) encodes a zinc finger protein predicted to interact with nucleic acids. Zinc finger proteins commonly function in transcriptional regulation and other processes involving DNA or RNA binding. Although the specific biological functions of ZNF101 remain incompletely characterized, its zinc finger domains suggest a potential role in regulating nucleic acid-dependent cellular processes, particularly gene expression.

A human liver tumor cell line established from the tumor tissue of a 15-year-old Caucasian male in 1975, originally described as a well-differentiated hepatocellular carcinoma and later reclassified by some sources as a hepatoblastoma. The cells produce multiple plasma proteins, including albumin, alpha-fetoprotein, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, and plasminogen. Hepatitis B virus surface antigens have not been detected, and the cells respond to stimulation with human growth hormone.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.