ZNF174 Knockout cell line (HCT-15)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZNF174 (zinc finger protein 174) encodes a nuclear zinc finger protein containing three C2H2-type zinc fingers, a putative nuclear localization signal, and an N-terminal SCAN domain that supports homodimerization. The protein is believed to function as a transcriptional repressor, suggesting a role in regulation of gene expression through sequence-specific or protein-mediated transcriptional control.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.