ZNF189 Knockout cell line (HCT-15)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZNF189 (zinc finger protein 189) encodes a Kruppel-like zinc finger protein containing C2H2-type DNA-binding zinc finger repeats. Members of this protein family can regulate gene expression through sequence-specific DNA binding, while KRAB domains found in many related proteins contribute to transcriptional repression. The specific molecular functions of ZNF189 remain incompletely characterized.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.